紫杉醇诱导胃癌细胞凋亡的实验研究

Empirical study on the apoptosis of gastric cancer cells induced by taxoi

目的探讨紫杉醇对胃癌细胞的诱导凋亡作用及其诱导的胃癌细胞凋亡的周期时相性。方法用Sub-G1法检测紫杉醇诱导胃癌细胞MKN-28的凋亡，API法检测紫杉醇诱导胃癌细胞凋亡的周期时相性，并分选后激光共聚焦显微镜技术（PSC）观察形态学，MTT法检测紫杉醇对临床胃癌组织细胞的敏感性。结果Sub-G1法结果显示紫杉醇能诱导胃癌细胞的凋亡，紫杉醇（浓度10mg／L）诱导胃癌细胞凋亡在10h后达到高峰；API法检测结果显示紫杉醇诱导胃癌细胞发生凋亡的时相在G2／M期，PSC形态学观察到G2／M期凋亡特征；MTT法结果显示紫杉醇诱导的20例临床胃癌组织标本中，有16例抑制率大于50％。结论紫杉醇能诱导胃癌细胞发生凋亡，相对较敏感，其诱导的胃癌细胞凋亡具有周期时相性。

Objective To investigate apoptosis of gastric cancer cells induced by taxol, and its specific apoptotic cell cycle phase. Methods The apoptosis rate of gastric cancer cell line MKN-28 induced by taxol was detected with Sub-G1 method. The specific apoptotic cell cycle phase was detected with API and PSC method. The sensitivity of 20 cases clinic gastric cancer specimens induced by taxol was detected with the method MTT. Results With the Sub-G1 method the MKN-28 cells were induced by 10μg/ml taxol, after 10 h, the apoptosis rate reached its top apex; with the API method and the PSC method, the specific apoptosis took place in G2/M phase; the chemotherapy sensitivity of 16 cases out of 20 cases clinic gastric cancer specimens exceed 50 % with the method MTT. Conclusion Taxol could induce gastric cancer ceils to apoptosis and the apoptosis takes place in GJM phase; Taxol is sensitive to clinic gastric cancer specimens.