IL-10-592A>C多态与宫颈癌遗传易感的相关性分析

Relationship between IL-10-592A>C promoter polymorphism and the susceptibility to cervical cancer.

目的研究白细胞介素10(IL-10)基因启动子区-592A>C单核苷酸多态性(SNP)与广东汉族妇女宫颈癌易感的关系。方法利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析技术,对70例宫颈癌患者和108名健康对照个体的IL-10-592A>C多态位点进行分型,比较各组间等位基因及基因型频率的分布,分析该多态位点与宫颈癌易感的相关性。结果AA、AC、CC基因型在病例组中的频率分别为50.0%、32.9%和17.1%,在对照组中分别是47.3%、40.7%和12.0%,两组间基因型频率分布差异无统计学意义(χ2=1.56,P=0.459);以AA基因型作为参照,AC和CC基因型均没有显著增加个体患宫颈癌的风险(OR=0.76,95%C I=0.39-1.48,P=0.421以及OR=1.35,95%C I=0.55-3.29,P=0.516)。结论IL-10基因基因启动子区-592A>C多态位点与广东汉族妇女人群宫颈癌的易感性无关。

Objective： To investigate the relationship between IL - 10 - 592A 〉 C promoter polymorphism and the susceptibility to cervical cancer in ethnic Han Cantonese population. Methods ： The genotypes of the IL - 10 - 592A 〉 C promoter polymorphism were determined in 70 cervical cancer patients and 108 healthy female blood donors by polymerase chain reaction - based restriction fragment length polymorphism （PCR- RFLP） . The genotype and allele frequency distribution were compared. Association between the polymorphism and the risk of cervical cancer was evaluated using unconditional logistic regression analysis. Results ： The frequency of AA, AC and CC genotypes in the case subjects were 50. 0% , 32. 9% and 17. 1% respectively and in the control subjects were 47. 3%, 40. 7% and 12. 0%. No significant difference was observed in the frequency of IL - 10 -592A 〉 C genotypes between cases and controis （ Х^2 = 1.56, P = 0. 459） . Unconditional logistic regression analysis revealed that the risk of cervical cancer was not significantly increased in both AC heterozygotes [ odds ratio （OR） = 0. 76, 95 % confidence interval （CI） = 0. 39 - 1.48, P =0. 421 ] and CC homozygotes （OR = 1.35, 95 % CI = 0. 55 -3.29, P =0. 516）, compared with AA homozygotes. Conclusion： IL - 10 -592A 〉 C promoter polymorphism is not associated with cervical cancer in ethnic Han Cantonese population.