胎儿生长受限患者胎盘组织中松弛素受体的表达

Expression of relaxin receptor in placental tissues of normal pregnancy and fetal growth restriction.

目的探讨胎儿生长受限(FGR)患者胎盘组织中松弛素受体LGR7表达与胎儿生长受限发病的关系。方法应用链霉菌抗生物素蛋白-过氧化物酶连接法和RT-PCR技术检测40例正常孕妇(正常组)及40例胎儿生长受限组(FGR组)胎盘组织中松弛素受体LGR7及mRNA的表达。结果(1)两组孕妇的胎盘组织中均有松弛素受体LGR7表达,主要定位于胎盘绒毛滋养细胞的细胞膜上,在细胞滋养细胞、合体滋养细胞中都有较高的表达水平。(2)正常组胎盘组织中松弛素受体LGR7蛋白的表达水平为0.928±0.002,FGR组为0.620±0.022,两组比较,差异有统计学意义(P<0.01)。(3)正常组胎盘组织中松弛素受体LGR7mRNA表达水平为0.780±0.021,FGR组组为0.414±0.032,两组比较,差异有统计学意义(P<0.01)。结论FGR患者胎盘组织中松弛素受体表达水平下降,可能与FGR的发病有关。

Objective： To study the location and level of relaxin receptor in placenta tissues of preeclampsia and normal pregnancy, and the relationship of relaxin receptor with fetal growth restriction （FGR） . Methods： Forty placenta tissue samples from pregnant women with FGR （study group）, and 40 samples from normal pregnancies （control group） were obtained. We detected the expression of relaxin receptor by immunohistochemistry and the expression of relaxin receptor mRNA by RT - PCR. Results ： In the placenta of control and FGR groups, the leucine - rich repeat - containing G protein - coupled receptor （ LGR7 ） was positively expressed. Relaxin receptor was located in the membrane of trophoblast cells. There were both strong signals on cytotrophoblastic ceils and syneytiotrophoblast. The level of relaxin receptor in the control group was 0. 928 ± 0. 002, and 0. 620 ± 0. 022 in FGR group. Thedifference between the two groups was obvious （ P 〈0. 01 ） . In the control group, the level of relaxin receptor mRNA was 0. 780 ± 0. 021 ; in FGR group, it was 0. 414 ± 0. 032. The difference was obvious （ P 〈 0. 01 ） . Conclusion： Decrease in the expression of relaxin receptor at placenta is related with the occurrence and development of FGR.