不同超排卵方案和小鼠品系来源的2-细胞期胚胎体外培养比较

The effects of superovulation and strains on 2-cell mouse embryos culture in vitro.

目的比较激素注射间隔时间差异对昆明系小白鼠(KM小鼠)超数排卵的影响以及KM小鼠和ICR小鼠2-细胞胚胎的体外发育能力。方法29只KM小鼠随机分成两组,分别间隔48h和72h注射10 IU的孕马血清(PMSG)和尿绒毛膜促性腺激素(HCG),比较获取2-细胞胚胎数以及其体外发育能力的差异;分7次同时常规超排KM小鼠和ICR小鼠,体外培养2-细胞胚胎,记录囊胚形成数。结果48h超排卵组获取2-细胞期小鼠胚胎数目显著多于72h超排卵组(P<0.01),但前者的囊胚形成率却显著低于后者,两者间差异有统计学意义(P<0.05)。KM小鼠和ICR小鼠2-细胞胚胎的囊胚形成率间无显著差异(P>0.05)。结论间隔48h超排KM小鼠可获取较多胚胎,但间隔72h超排所获2-细胞期小鼠胚胎体外发育潜能好;小鼠品系不影响2-细胞胚胎的体外培养结果。

Objective： Compared the methods of superovulation and the development of KM mouse and ICR mouse 2 - cell embryos. Methods ： 29 KM mice were divided into two groups, after 48h or 72h of given PMSG, hCG was injected, and the 2 - cell embryos were cultured in vitro. After that, ICR mouse and KM mouse were superovulated according to the conventional method, and the 2 -cell embryos were cultured for 72h. Results： The numbers of KM mouse embryos and the percentage of KM mouse blastocysts were significant difference between 48h and 72h （ P 〈0. 05） . The group of 48h had the more number of embryos but the lower percentage of blastocysts. The difference of the percentage of blastocysts between KM mouse and ICR mouse wasn＇t significance （ P 〉 0. 05 ） . Conclusion ： The interval of injected PMSG and HCG will affect the number and the development of mouse embryos. The development in vitro of 2 -cell embryos is same between KM mouse and ICR mouse.