阿斯巴甜合成菌株的初步筛选

Primary screening of aspartame synthesizing strains

从富含蛋白的土样中筛选能分解二肽甜味剂——阿斯巴甜的菌种,利用其可逆合成二肽的原理,以L-天冬氨酸和L-苯丙氨酸甲酯为底物一步合成阿斯巴甜,初步探讨微生物一步酶法合成阿斯巴甜的生物合成工艺。经过高效液相检测,结果表明初步筛选到一株能合成阿斯巴甜的菌株APM-1,为今后利用微生物一步法合成阿斯巴甜打下基础。

The strains for hydrolyzing aspartame were screened from abundant protein soil.Based on the protease catalyse reverse reaction.L-aspartic and L-phenylalanine methyl ester were cataltzed by the protease for synthesizing aspartame,and one step biosynthesis technology for synthesizing aspartame was investigated.The results showed that the strains APM-1 for synthesizing aspartame were obtained by the HPLC.This result will establish the base for one step biosynthesis technology for synthesizing aspartame by using microorganism.