摘要
以东北龙胆茎段为外植体,以MS为基本培养基,添加不同浓度的BA及NAA,筛选诱导不定芽及生根的最佳培养基。结果表明:以70%酒精处理60 s+0.1%升汞处理10 min,成活率可达97.5%;以MS+BA 3.0 mg/L+NAA 0.1 mg/L为不定芽诱导培养基,诱导率可达94.44%,试管苗在1/2MS+IBA 0.5 mg/L生根培养基平均生根率为94.74%。
Stem segements of Gentiana rnanshuica Kitagawa were used as explants with MS as the basic medium, Add with different concentration BA and NAA, the best medium of induction on adventitious bud and rooting were selected. The results showed that the optimum disinfector was 70 %alcohol for 60 seconds and HgCl2 for 10miutes.The optimal medium for inducing adventitious bud was MS＋BA 3. 0 mg/L＋NAA 0. 1 mg/L and the ratio of induction was 94. 44%. The rooting Culture medium was 1/2MS＋IBA 0.5 mg/L and the ratio of rooting was 94. 74%.
出处
《北方园艺》
CAS
北大核心
2010年第5期148-150,共3页
Northern Horticulture
关键词
东北龙胆
茎段
组织培养
Gentiana manshuica Kitagaw
stem segement
tissue culture
