阿司匹林抵抗与血小板膜糖蛋白Ⅰbα基因多态性的关系

Relationship of VNTR Polymorphism of Platelet Glycoprotein I bα to Aspirin Resistance

目的研究阿司匹林抵抗者血小板膜糖蛋白Ⅰbα(GPⅠbα)基因VNTR多态性的等位基因及基因型分布状况。方法选择110例服用阿司匹林(100mg/d)至少7d以上的心脑血管疾病患者,其中阿司匹林半抵抗组10例,阿司匹林敏感组100例。另选择31例健康者作为健康对照组。采用二磷酸腺苷(ADP)和花生四烯酸(AA)作为诱导剂,在血小板聚集仪上测定3组血小板最大聚集率。采用PCR扩增及电泳技术进行VNTR多态性分型。结果本组患者中阿司匹林半抵抗发生率为9.1%。VNTR基因多态性测定结果显示,CC基因型在阿司匹林半抵抗组和阿司匹林敏感组的分布频率差异有统计学意义(χ2=4.1301,P=0.0421),BB、BC、BD、CD、DD基因型分布与阿司匹林抵抗无相关性,但是BC、BD基因型在阿司匹林抵抗组中的分布有增高趋势。结论VNTR中CC基因型的人群较少发生阿司匹林抵抗。

Objective To study the relationship between the presence of different VNTR alleles of GPⅠbα and the frequency of aspirin resistance.Methods One hundred ten cardio-cerebral vascular disease patients receiving aspirin （100 mg/d） for at least 7 d were divided into groups aspirin resistance （n=10） and aspirin sensitive （n=100）,and another 31 healthy subjects were enrolled as control group.Using adenosine diphosphate （ADP） and arachidonic acid （AA） as inducer,maximum platelet aggregation rate was detected on platelet aggregation instrument.PCR amplification and electrophoresis technique was used to conduct VNTR polymorphism typing.Results The occurrence of the CC allele was significantly higher in patients with aspirin sensitivity than in those with aspirin resistance（P〈0.05）.Conclusion CC homozygosity is associated with good response to aspirin therapy.