HPLC-MS/MS法测定岩黄连提取物中脱氢卡维丁的含量

Determination of dehydrocavidine in extractions of Corydalis saxicola Bunting by HPLC-MS/MS

采用高效液相色谱电喷雾离子化质谱法分离并测定岩黄连提取物中脱氢卡维丁的含量．色谱采用XTerra MS C18柱（100mm×2．1mm，5μm），以0．1％甲酸水溶液-甲醇为流动相，梯度洗脱，流速为0．2mL/min．质谱采用三重四级杆的质谱仪，电喷雾离子源，正离子扫描方式，以多反应离子监测（MRM）模式进行定量检测．结果表明，脱氢卡维丁在0．2～200ng／mL（r=0．9995）范围内呈良好线性关系，平均加样回收率为99．29％，相对标准偏差（RSD）为2．4％．

To establish a high performance liquid chromatography-mass spectrometry/mass spectrometry （HPLC -MS/MS） method for the content determination of dehydrocavidine in the extractions of Corydalis saxicola Bunting. HPLC was performed on the XTerra MS Cls column（ 100 mm ×2. 1 mm,5 μm） with the 0.1% formate-acid solution-methanol as the mobile phase. The flow rate was 0.2 mL/min with gradient elution. The mass spectrometry detection was performed on the eletronspray tandem mass spectrum in the positive mode and operated in positive-ionization mode, and multiple reaction monitor was used to detect the granisetron concentration. The result indicated that dehydrocavidine had a linear relationship within the range of 0.2 - 200 ng/mL（ r = 0. 999 5 ）. The average recovery was 99.29% and RSD was 2.4%.