基于遗传算法的光合细菌过氧化物酶电泳分析方法的建立

Establishment of Gel Electrophoresis for the Analysis of Peroxidases in Photosynthetic Bacteria Based on Genetic Algorithm

建立光合细菌中过氧化物酶的酸性聚丙烯酰胺凝胶电泳(A-PAGE)分析方法。以电泳图谱的谱带数和灰度为指标,运用正交试验和遗传算法对该方法的电泳条件进行优化。实验结果表明,当凝胶中Triton X-100含量为0.31%、菌体破碎时间为19min、样品中Triton X-100含量为0.79%时,可获得清晰的沼泽红假单胞菌过氧化物酶图谱;当凝胶中Triton X-100含量为0.27%、菌体破碎时间为16min、样品中Triton X-100含量为0.76%时,可获得清晰的球形红细菌过氧化物酶图谱。本方法为光合细菌中过氧化物酶的分析提供了一种新的、简单、快速的手段。

To establish the acid polyacrylamide gel electrophoresis （A-PAGE） analyzing method of peroxidase isoenzymes in photosynthetic bacteria.According to the number and the grey scale of bands,the conditions of the A-PAGE were optimized by the orthogonal design and the genetic algorithm.The results indicated that when the Triton X-100 concentration in the gels was 0.31%,the ultrasonic time was 19 min,the Triton X-100 concentration in the samples was 0.79%,the clear peroxidase isoenzyme zymograms of Rhodopseudomonas palustris were obtained,when the Triton X-100 concentration in the gels was 0.27%,the ultrasonic time was 16 min,the Triton X-100 concentration in the samples was 0.76%,the clear peroxidase isoenzyme zymograms of Rhodobacter sphaeroides were obtained.The paper set up a simple and rapid method for the analysis of peroxidase isoenzymes in photosynthetic bacteria.