奶粉-三聚氰胺的荧光光谱特性研究

Study on the fluorescent spectral characteristics of milk powder and melamine

为了研究奶粉、三聚氰胺、奶粉-三聚氰胺混合水溶液的稳态荧光光谱和同步荧光光谱特征,采用280nm的激励光照射奶粉-水溶液时,能发射峰值位于330nm的荧光。利用恒波长同步荧光分析技术研究了该混合溶液的光谱特性,分别改变不同的波长差Δλ,当Δλ=60nm时,同步荧光强度达到最大;混合溶液中奶粉的浓度改变时,其同步荧光强度和浓度间满足线性关系。同时得到了奶粉和三聚氰胺混合溶液的同步荧光光谱,当Δλ=38nm时,奶粉同步荧光峰位位于283nm,三聚氰胺位于319nm,奶粉-三聚氰胺混合溶液中两个峰位均存在。结果表明,利用同步荧光分析技术可以较好地检验出奶粉中是否含有三聚氰胺。该研究能为三聚氰胺及其衍生物的物质特性研究提供参考,并为三聚氰胺的含量检测提供帮助。

In order to obtain the steady state fluorescent and synchronous fluorescent spectral characteristics of milk powder, melamine and the mixture of milk powder-melamine respectively, UV light of 28Onto was chosen as the excitation light to irradiate milk powder-water solution and the emission peak was at 330nm in the fluorescent spectrum. The constant-wavelength AA was used in synchronous fluorescence spectrometry. The experiment results reveal while changing the value of △λ, the synchronous fluorescent intensity altered, and it reached maximum when △λ= 60nm. Scanning the synchronous fluorescence of different milk powder-water solutions with △λ = 60nm, the fluorescent intensity changed and the relationship between the fluorescence intensity and the concentration of the milk powder-water solution was linear. When △λ = 38nm, the synchronous fluorescent spectra of milk powder, melamine and the mixture of melamine and milk powder were obtained. The experiment results indicated that the synchronous fluorescent peak of milk powder was at 283nm, for melamine, it was at 319nm. In the milk powder-melamine solutions, there were two peaks of 283nm for milk powder and 319nm for melamine, so that whether there was melamine in the milk powder can be determined. The research may contribute to the research of the characteristics of melamine and its derivatives and the detection of melamine in the milk.