海南霸王岭热带雨林常见植物丛枝菌根真菌调查

Arbuscular mycorrhizal fungi associated with common tree species in a tropical rain forest in Bawangling of Hainan Island,China

对海南霸王岭热带雨林的12科16种常见植物的丛枝菌根状况进行了调查,用碱解离-酸性品红染色法进行了真菌鉴定。结果表明,13种植物形成典型的丛枝菌根,占所调查植物的81%;3种植物没有形成丛枝菌根,占所调查植物的19%。用湿筛沉淀法从这些植物根际土壤中共分离鉴定出了3属11种丛枝菌根真菌(AMF),即无梗囊霉属(Acaulos-pora)3种,球囊霉属(Glomus)7种,巨孢囊霉属(Gigaspora)1种;其中,球囊霉属是样地的优势属。在AMF中,孔窝无梗囊霉(A.foveata)分离频率最高,在14种植物的根际土中都有发现;此外,大果球囊霉(G.macrocarpum)的相对多度最大,为59%,具有最强的产孢能力。同时,在11种植物的根中发现了深色有隔内生真菌(DSE),占调查植物的69%;其中,11种植物同时被DSE和AMF感染。

An investigation was made on the arbuscular mycorrhiza （AM） and dark septate endophytes （DSE） associated with 16 common plant species belonging to 12 families in a tropical rain forest in Bawangling of Hainan Island,China. The fungi were identified by alkaline lysising and acid fuchsin staining. Among the surveyed plant species,13 of them （occupying 81% of the total） were typically colonized by arbuscular mycorrhizal fungi （AMF）,while 3 species （about 19%） did not form AM. Eleven AMF species in 3 genera were isolated from the rhizosphere soils by wet-sieving,of which,3 species came from Acaulospora,7 species from Glomus,and 1 species from Gigaspora. Glomus was the dominant genus. A. foveata was the most common species,which was found in the rhizosphere soils of 14 plant species. G. macrocarpum had the highest ability to produce spores,and its relative abundance was 59%. Eleven plant species （about 69% of the total） were found to be colonized by DSE,occupying 69% of the total; and 11 plant species were simultaneously colonized by AMF and DSE.