基于线粒体DNA D-loop区序列的我国灰鹅遗传多样性和起源分析

Genetic diversity and origins of Chinese native greylag goose breeds based on mtDNA D-loop sequences

为了探究我国灰鹅品种的遗传多样性和系统进化,运用DNA测序技术测定了我国13个灰鹅品种mtDNAD-loop区521bp序列。结果表明:这13个灰鹅品种521bpD-loop区序列的T、C、A和G碱基含量分别为23.8%、29.0%、32.3%和15.0%;平均单倍型多样度和核苷酸多样度分别为0.19245和0.00036;13个灰鹅种间变异大于种内变异,且均未出现群体扩张现象;共享单倍型和系统进化树分析表明,伊犁鹅起源于灰雁(Anser anser),其余12个灰鹅品种起源于鸿雁(A.cygnoides)。

In order to reveal the genetic diversity and systematic evolution of Chinese greylag goose breeds,the 521 bp control region（D-loop）of mitochondrial DNA from 13 greylag goose breeds were sequenced.In the D-loop,the contents of T,C,A,and G nucleotides occupied 23.8%,29.0%,32.3%,and 15.0%,and the average haplotype diversity（Hd）and nucleotide diversity（Pi）were 0.19245 and 0.00036,respectively.The nucleotide variance value among the populations was bigger than that within the populations,and no population expansion was observed for the breeds.The shared haplotype analysis and systemic evolution analysis revealed that the 13 breeds had two maternal origins,among which,Yili breed originated from greylag goose（Anser anser）,and the other 12 breeds originated from swan goose（A.cygnoides）.