非抑制电导阳离子色谱法测定牛奶中的碳酸胍

Determination of guanidine carbonate in milk by non-suppressed conductivity cation chromatography

目的：建立一种以MSA（甲基磺酸）和乙腈作混合淋洗液,用离子交换色谱柱和非抑制电导检测器检测,测定牛奶中的碳酸胍的检测方法。方法：考察了在不同有机改进剂-乙腈的浓度下,碳酸胍的分离程度。结果：该方法具有准确、快速、高效的特点。碳酸胍在1.0-50μg/ml浓度范围内线性良好,相关系数为0.9999,回收率为99.20%。结论：该方法操作简单,结果准确,可作为检测牛奶中的碳酸胍的通用方法。

Objective：To establish a sensitive method for the determination of guanidine carbonate in milk by ion chromatography（IC） with non-suppressed conductivity detection.Methods：To guanidine carbonate was isolated by using the different concentration of acetonitrile（ACN） as organic modifier.Results：This method was accurate,fast and efficient.The linear range was 1.0-50.0 μg/ml for guanidine carbonate,the correlation coefficients was 0.9999.The recoveries was 99.20% for guanidine carbonate.Conclusion：The developed method is satisfied with the residual determination for guanidine carbonate in milk.