莱克多巴胺酶联免疫分析方法的建立

Development of an ELISA for ractopamine

目的：建立莱克多巴胺酶联免疫分析方法。方法：采用混合酸酐法将莱克多巴胺与牛血清白蛋白偶联,并免疫新西兰白兔制备抗莱克多巴胺抗血清。以间接ELISA法测定血清效价,测得抗血清最佳工作浓度为1：8000,该抗莱克多巴胺抗血清对克伦特罗、沙丁胺醇等无交叉反应性。结果：在优化的条件下,针对莱克多巴胺的酶联免疫分析方法,最低检测限为0.1 ng/ml,其检测范围为0.04-25 ng/ml。结论：该方法快速、简单、准确,可以用于检测食品中莱克多巴胺的残留量。

Objective：To develop an ELISA method for analysis of ractopamine.Methods：The complete antigen was made after ractopamine was reacted with succinic anhydride and isobutychloroformate and was conjugated with BSA.The BSA conjugate was used to immunize new zealand rabbits and polyclonal antibodies against ractopamine were obtained.Results：An indirect enzyme-linked immunoassay was developed,and the optional dilution of the anti-RAC serum was 1：8000 and anti-RAC serum has not the cross-reactivity with salbutamol and clenbuterol.The limit of detection for RAC was 0.1 ng/ml in the range of detection 0.04 ng/ml～25 ng/ml.Conclusion：This method is rapid,simple and precise,and can be applied to analyse residual ractopamine in food.