HIV/AIDS患者T淋巴细胞HLA-DR抗原表达分析

Study on expression of HLA-DR antigen of T-Lymphocyte in HIV/AIDS Patients

目的:研究HIV/AIDS患者外周血T淋巴细胞HLA-DR抗原的表达,探讨其临床意义。方法:用流式细胞仪检测71例HIV/AIDS患者和40例健康体检者外周血T淋巴细胞亚群及各亚群HLA-DR抗原百分比(HLA-DR+CD3+/CD3+、HLA-DR+CD4+/CD4+、HLA-DR+CD8+/CD8+)。结果:①与对照组相比,HIV组和AIDS组的CD3细胞、CD4细胞、CD8细胞HLA-DR抗原百分比均显著高于对照组;AIDS组的CD3细胞、CD4细胞HLA-DR抗原百分比均显著高于HIV组。②HIV/AIDS患者CD3细胞、CD4细胞HLA-DR抗原百分比均与CD4细胞数负相关,相关系数分别为r=-0.392(P<0.01)、r=-0.619(P<0.01)。结论:HIV/AIDS患者T淋巴细胞持续紊乱,测定T淋巴细胞HLA-DR抗原表达有助于评价HIV/AIDS患者的免疫状况以及疾病的进程。

Objective：To study the expression of HLA-DR antigen of T-Lymphocyte and their clinical significance in HIV/AIDS Patients.Methods：The percentages of the HLA-DR antigen and subsets of T-Lymphocyte were detected by flow cytometry in HIV/AIDS patients（n=71） and normal controls（n=40）.Results：Compared with the normal group,all of the percentages of the HLA-DR antigen in HIV and AIDS group was significantly increased,the percentages of CD3、CD4 cells of the HLA-DR antigen in AIDS group were higher than those in HIV group.The percentages of CD3,CD4 cells of the HLA-DR antigen in HIV and AIDS patients were negatively correlated with CD4 count.Conclusion：T lymphocytes are sustained for disorder in HIV/AIDS patients.To understand the HLA-DR antigen expression of the T-lymphocytes in HIV/AIDS patients may help to evaluate the patients′ immune status and the clinical disease progression.