不同产地白芷中欧前胡素含量及HPLC指纹图谱的对比研究

Study on the Content of Imperatorin and HPLC Fingerprint of Baizhi Collected from Different Areas

目的:比较不同产地白芷中欧前胡素含量的差异,同时对不同产地白芷药材进行HPLC指纹图谱对比研究。方法:收集7省市15个白芷主产区对口药材及种子,并将种子种植在四川遂宁白芷种质资源圃中,于次年正常采收期采挖、干燥,对其及与之相对应的白芷对口药材进行欧前胡素含量测定及HPLC指纹图谱对比研究。结果:不同产地白芷中欧前胡素含量存在差异;不同产地样品栽种于同一环境后,欧前胡素含量整体有明显提高;化学表达也随产地及栽培方法的一致而趋同。结论:白芷中香豆素类成分与产地环境密切相关,产地生境及栽培技术可能是决定其质量的关键因素。

Objective：To compare the content of imperatorin and HPLC fingerprint of Baizhi collected from different areas.Methods：The medicinal materials and seeds of Baizhi from 15 main habitats of 7 provinces and cities were collected,and the seeds were planted in the germplasm resource garden of Suining,Sichuan.The content of imperatorin and HPLC fingerprint were compared using the samples collected from different areas and germplasm resource garden collected and dried at the most suitable harvest date.Results：The differences on the content of imperatorin and HPLC fingerprint were obvious.The content of imperatorin significantly increased and the HPLC fingerprint were trending to coincide when the herbs were planted in the same place.Conclusion：The environmental factors and the cultivation technique had great influences on the quality of Baizhi.