淡竹叶药材HPLC指纹图谱研究

Study on Fingerprints Spectrum of Lophatherum gracile by HPLC

目的:采用HPLC法建立淡竹叶药材的指纹图谱。方法:采用Agilent Eclipse XDB-C18(150 mm×4.6mm,5μm)色谱柱;以甲醇(A)-3%冰乙酸(B)为流动相,进行梯度洗脱;采用波长切换法检测;流速:1.0 mL/min;柱温:30℃。结果:检测了不同来源15批淡竹叶药材,标定了16个共有峰,相似度较高,各色谱峰分离度较好,达到了中药指纹图谱研究的技术要求。结论:本法准确、可靠,建立的HPLC指纹图谱为淡竹叶的质量控制提供更全面的信息。

Objective：To establish the fingerprint spectrum of Lophatherum gracile by HPLC.Methods： The samples were separated on Agilent Eclipse XDB-C18 column（150 mm×4.6 mm,5 μm）with methanol（A）-3% acetic acid（B）as the mobile phase at a flow rate of 1.0 mL/min at 30 centigrades,adopting the gradient elution process with switching the Wavelength.Results：15 samples of different origin Lophatherum gracile Brongn were detected and 16 peaks in the chromatogram were common.there was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.Conclusion： The method is accurate,reliable and the fingerprint spectrum provides more information for controlling the quality of Lophatherum gracile.