鹿衔草总黄酮对异丙肾上腺素诱导的大鼠急性心肌缺血的保护作用

Protective Effect of Total Flavonoid of Herba Pyrolae on Acute Myocardial Ischemic Injury Induced by Isoproterenol in Rats

目的:观察鹿衔草总黄酮对异丙肾上腺素诱导的大鼠急性心肌缺血的保护作用并初步探讨其作用机制。方法:皮下多点注射异丙肾上腺素造成大鼠急性心肌缺血模型,记录皮下注射异丙肾上腺素后ECG,测定血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)的活性及血清丙二醛(MDA)、一氧化氮(NO)、游离脂肪酸(FFA)的含量,测定心脏指数(HW/BW),观察心肌组织病理学改变。结果:鹿衔草总黄酮能减轻异丙肾上腺素诱导的大鼠心电图T波降低;降低血清CK、LDH的活性及FFA含量,降低心脏指数,减轻心肌损伤及坏死的程度;升高血清SOD活性及NO含量,降低血清MDA含量。结论:鹿衔草总黄酮对异丙肾上腺素诱导的大鼠急性心肌缺血具有保护作用,其机制可能与抗脂质过氧化、增加NO的生成和释放有关。

Objective：To investigate the protective effect of total flavonoid of Herba Pyrolae（TFHP）on acute myocardial ischemic injury induced by isoproterenol in rats and the primary mechanisms.Methods：Acute myocardial ischemic models were established by i.s.of isoproterenol.ECG of the rats were recorded,activities of creatine phosphokinase（CK）,lactate dehydrogenase（LDH）and the superoxide dismutase（SOD）,and the levels of malondialdehyde（MDA）,NO and FFA in rat serum were determined,heart index were measured and histopathological changes of myocardium were observed.Results：In comparison with model group,TFHP raised the height of T wave,reduced CK,LDH activities and FFA levels in serum,decreased heart index and relieved myocardial ischemic injury.The primary study of its mechanism showed that TFHP decreased MDA level,increased SOD activity and NO levels in the rat serum.Conclusion：TFHP has protective effect on acute myocardial ischemic injury possibly via antioxidation and increasing the release and production of NO.