芒果苷对白血病HL-60细胞周期及CDC2/CyclinB1表达的影响

Effects of Mangiferin on Cell Cycle Status and CDC2/CyclinB1 Expression of HL-60 Cells

目的:观察芒果苷对白血病HL-60细胞周期分布及细胞周期素B1(CyclinB1)、细胞周期分裂基因2(CDC2)表达的影响,以进一步探讨芒果苷抗白血病的分子作用机制。方法:应用MTT比色法测定芒果苷对白血病HL-60细胞增殖的抑制作用;用流式细胞术(FCM)检测芒果苷对细胞周期的影响;用RT-PCR技术检测CyclinB1及CDC2 mRNA表达水平。结果:芒果苷呈剂量及时间依赖性抑制HL-60细胞的生长,作用24 h后,G2/M期细胞增多,出现G2/M期阻滞;CyclinB1 mRNA和CDC2 mRNA的表达随药物质量浓度的增加而逐渐增强,在芒果苷质量浓度80μmol/L时表达达到峰值。结论:芒果苷可抑制HL-60细胞增殖,阻滞细胞周期于G2/M期,显著上调HL-60细胞CyclinB1 mRNA和CDC2 mRNA的表达水平。芒果苷诱导HL-60细胞G2/M期阻滞可能是其抗白血病作用的分子机制之一。

Objective：To study the effects of mangiferin on cell cycle status and CDC2/CycliB1 expression in HL-60 cells, and its melocular mechanism for treating leukemia Methods ： The effect of Mangiferin on HL-60 cells proliferation was determined by MTT assay;The change of cell cycle status in HL-60 cells treated with mangiferin was performed by the flow cytometry;The expressions of CDC2 mRNA and CyclinB1 mRNA were detected by semiquantitative RT-PCR. Results：The growth inhibition effects of mangiferin in HL-60 cells were enhanced as the mangiferin concentration increased and exposure time prolonged; HL-60 ceils in G2/M phase increased in a dose-dependent way 24 hours after mangiferin admininistration,indicating G2/M phase blockage;the expressions of CDC2 mRNA and CyclinB1 mRNA enhanced in a dose-dependent way and came to the peak at 80 μmol/L mangiferin. Conclusion ： Mangiferin can inhibit the proliferation of HL-60,block the cell cycle progression in G2/M phase,and it can significantly increase the expressions of CDC2 mRNA and CyclinB1 mRNA of HL-60 ceils. G2/M phase blockage may be one of its molecular mechanism for treating leukemia.