六味地黄丸对庆大霉素肾中毒大鼠肾核因子κB的影响

Effect of Liuwei Dihuang Pill on Expression of NF-κB in Kidney Lesion Induced by Gentamicin in Rats

目的:观察六味地黄丸对大鼠庆大霉素肾毒性的疗效并初步探索其作用机制。方法:将大鼠随机分为3组:正常对照组(C)、模型组(GM)、六味地黄丸组(LDW)。采用生化检测和蛋白免疫印迹方法观察各组动物肾功能、氧化产物及酶类的变化及用药对信号通路分子p38、核因子κB(NF-κB)蛋白表达的影响。结果:与正常对照组比较,GM组大鼠体质量显著下降,肾脏指数、肾功能、肾皮质氧化产物MDA含量与NOS酶活性显著增高,SOD酶活性显著降低,NF-κB蛋白表达显著增多。与GM组比较,治疗第1周末LDW组大鼠肾功能、肾皮质NOS活性显著降低,SOD活性显著增高,NF-κB蛋白表达显著降低。结论:六味地黄丸能通过调节NF-κB的表达改善庆大霉素所致肾脏氧化损伤,对庆大霉素肾中毒具有一定疗效。

Objective：To study the effect of Liuwei Dihuang Pill on kidney lesion in rats induced by gentamicin and its mechanism. Methods：Rats were divided into three groups： control group, gentamicin group, Liuwei Dihuang Pill group randomly. Parameters of kidney function and oxidative production in three groups were tested by biochemical analysis. Nuclear Faetor-κB （ NF-κB ） protein were determined by western blotting. Results ： Parameters of weight, kidney indexes, oxidative production MDA and enzyme activity of NOS in kidney cortex of gentamiein group significantly increased （P 〈 0. 05 ） , and enzyme activity of SOD significantly decreased （P 〈 0. 05 ）. The protein expression of NF-κB in gentamicin group was significantly up-regulated. After treatment with Liuwei Dihuang Pill, the above-mentioned up-regulated parameters were all significantly inhibited. Conclusion：Liuwei Dihuang Pill can inhibit the expression of NF-κB protein, so as to alleviate the renal oxidative lesion, and it may be one of the important mechanisms for treating gentamicin induced kidney lesion.