新型鱼腥草素衍生物对TNF-α诱导的NI H3T3细胞增殖及Syndecan-4蛋白表达的影响

Effects of A New Houttuyfonate Eerivative on Proliferation of NIH3T3 Cell and Expression of Syndecan-4 Induced by Tumor Necrosis Factor α

目的:观察一种新型鱼腥草素衍生物(new houttuyfonate derivative,NHD)对肿瘤坏死因子α(tumor necro-sis factorα,TNF-α)诱导的NIH3T3细胞增殖及Syndecan-4蛋白表达的影响。方法:以终浓度分别为TNF-α20 ng/mL、NHD 4μg/mL、NHD 8μg/mL、TNF-α20 ng/mL+NHD 4μg/mL及TNF-α20 ng/mL+NHD 8μg/mL对体外培养的NIH3T3细胞作用24 h,并设立相关对照组,用MTS/PMS法检测NIH3T3细胞的增殖,用Western blot蛋白免疫印迹法测定Syndecan-4蛋白的表达情况。结果:统计分析显示,与对照组比较,NHD各剂量单独应用对NIH3T3细胞的增殖无明显作用(P>0.05);NHD 4、8μg/mL可分别抑制TNF-α对NIH3T3细胞的增殖作用(P<0.05)。Western blot测定显示:与对照组比较,NHD各剂量单独应用对Syndecan-4蛋白的表达无明显影响(P>0.05),NHD4、8μg/mL均可抑制TNF-α诱导的Syndecan-4蛋白的表达(P<0.05)。结论:NHD对TNF-α诱导的NIH3T3细胞增殖及Syndecan-4蛋白表达均有抑制作用。

Objective： To investigate the effect of a new houttuyfonate derivative（NHD） on proliferation of NIH3T3 cell and expression of Syndecan-4 induced by TNF-α in vitro.Methods： NIH3T3 cells were cultured and exposed to TNF-α or NHD respectively,and then cotreated with TNF-α and NHD.All the groups were cultured for 24 hour in vitro,in addition to the untreated control group established for comparison.The ratio of proliferation of NIH3T3 cell was determined by non-radioactive MTS/PMS assay and the expression of Syndecan-4 was evaluated by western blot using anti-Syndecan-4 antibody.Results：Statistical analysis showed that,compared with the control group,NHD had no effect on VSMCs growth,but significantly inhibited NIH3T3 cell proliferation while induced by TNF-α.It also showed that compared with control group,NHD had no effect on the expression of Syndecan-4,but significantly inhibited its expression while induced by TNF-α（P〈0.05）.Conclusions：NHD can inhibit the proliferation of NIH3T3 cell and the expression of syndean-4 protein induced by TNF-α in vitro.