三七根及花总皂苷抗肿瘤细胞诱导的血小板聚集研究

Effect of Total Saponins from Rhizomes and Flowers of Panax notoginseng on Tumor Cell Induced Platelet Aggregation

目的:考察三七根和花总皂苷抗肿瘤细胞诱导的血小板聚集(TC IPA)作用。方法:应用B ron氏比浊法分别从体外、体内考察三七根和花总皂苷对人乳腺癌细胞株MDA-MB-231诱导的血小板聚集能力。结果:三七花总皂苷在120、240、480 mg/L质量浓度下对TC IPA均有一定的抑制作用,三七根总皂苷只在高、中剂量抑制TC IPA。体内实验三七根和三七花总皂苷各剂量均可显著抑制TC IPA,且呈剂量依赖关系。三七花作用效果优于三七根。结论:三七根和花总皂苷具有抗肿瘤细胞诱导的血小板聚集能力,具有潜在的抗肿瘤血行转移能力。

Objective： To investigate the effects of total saponins from rhizomes and flowers of Panax notoginseng on tumor cell induced platelet aggregation（TCIPA）.Methods： MDA-MB-231 breast carcinoma was applied as inductor and the platelet aggregation were investigated by Born′s method in vitro and in vivo.Results： Saponins from the flowers of Panax notoginseng inhibited TCIPA at the dose of 120,240,480 mg/L respectively in vitro and in vivo.Saponins from rhizomes of Panax notoginseng inhibited TCIPA only at its high dose in vivo.Conclusion： The saponins from Panax notoginseng has a dose-dependent inhibition of TCIPA.It may be a new class of antimetastatic agent.