二期发色底物法外周血单核细胞促凝活性测定

Two stage chromogenic substrate assay for determination of procoagulant activity of periperal blood monocyte

以国产标化组织凝血活酶为代标准液，依据外源凝血途径建立反应系统。FXa水解发色底物释放硝基苯胺，于405um测定所得的吸光度与标准液中组织凝血活酶含量呈良好的观对数线性关系（r一0．998）。此方法测定范围在1～10°凝血活酶单位之间；标准曲线的重复性好（CV-5％）。经PBMC标本测定显示，应用本方法检测PBMCPCA符合实验要求。

Based on the extrinsic coagulation pathway, we established the reaction system using thromboplastinmade in our country as standard. The lysis of FXa chromogenic substrate (S2222) t0 release PNA, and thedose-response curve with thromboplastin showed a double-logarithmic correlation between optical density405 nm and thromboplastin concentration (r= 0. 998). The recommended working range was between 1 ~los TU. The reliability of standard curve was studied, CV were 5 %. Results indicated that the methodcan be used as an assay for determination of periperal blood monocyte (PBMC ) procoagulant activity(PCA ).