流式细胞术检测体液T淋巴细胞亚群的临床应用

Investigation of T lymphocyte subsets in fluid of the patients by flow cytometry

目的探讨胸水、腹水、肺泡灌洗液T淋巴细胞亚群的检测方法及临床价值。方法采用流式细胞术检测良、恶性疾病患者的50例胸水、46例腹水、47例肺泡灌洗液(BALF)中T淋巴细胞亚群的水平并分组进行比较。结果 1)胸水:结核性胸膜炎组胸水Ts细胞占T淋巴细胞的(27.36±6.41)%、Th细胞占T淋巴细胞的(67.98±7.41)%、Th/Ts比值为2.60±0.64,肺部恶性肿瘤组胸水Ts细胞占T淋巴细胞的(67.97±4.20)%、Th细胞占T淋巴细胞的(37.61±4.80)%、Th/Ts比值为0.47±0.10,与结核性胸膜炎组比较,恶性组Th、Th/Ts比值显著下降(P<0.01)而Ts显著升高(P<0.01);2)肺泡灌洗液:肺良性疾病组肺泡灌洗液Ts细胞占T淋巴细胞的(44.54±9.56)%、Th细胞占T淋巴细胞的(49.28±12.88)%、Th/Ts比值为1.13±0.29,肺部恶性肿瘤组肺泡灌洗液Ts细胞占T淋巴细胞的(65.11±7.77)%、Th细胞占T淋巴细胞的(34.57±6.65)%、Th/Ts比值为0.54±0.19,与良性病变组比较,恶性组Th下降(P<0.05),Th/Ts比值显著下降(P<0.01),而Ts显著升高(P<0.01)。3)腹水:结核性腹膜炎组腹水Ts细胞占T淋巴细胞的(33.11±3.34)%、Th细胞占T淋巴细胞的(66.69±3.78)%、Th/Ts比值为2.03±0.28,恶性组腹水Ts细胞占T淋巴细胞的(56.64±9.34)%、Th细胞占T淋巴细胞的(35.86±3.30)%、Th/Ts比值为0.65±0.10,与结核性腹膜炎组比较,恶性组Th、Th/Ts比值显著下降(P<0.01),而Ts显著升高(P<0.01)。结论胸腹水、肺泡灌洗液T淋巴细胞亚群检测可用于分析性质并有助于评估患者局部免疫功能状态。

Objective To evaluate the method of flow cytornetric analysis of T lymphocyte subsets in bodyfluid of the patients. Methods In ovder to get T lymphocytes by 3-colour staining method utilizing SSC/CD3Percp antibody,47 bronchoalveolar tavage fluid and 50 were tested by flow cytometery. Results 1 ）The percentage of Th is （67.98± 7.41） % ,Ts（27.36±6.41） % and Th/Ts（2.60±0.64）in BALF with lung cancer,and the percentage of Th is （37.61±4.8）%,Ts（67.97±4.20）%,and Th/Ts（0. 47±0.10）in BALF with lung cancer respectively, and the Th cells in BAI.F with lung cancer were lower than those in the benign pulmonary disorder（P〈0.05）, and the Th/Ts ratio was significantly lower while the Ts cells were significantly higher in BALF of lung cancer（P〈0.01）, comparied with the benign pulmonary disorder; 2）The percentage of Th is （35.76±7.71）% and Ts（64.31±7.37）% and Th/Ts（0.57±0. 18） in BALF with lung cancer, and the percentage of Th is（49. 27± 12.88）%,Ts（44.54±9. 56） %,and Th/Ts is （1.13± 0.29） in BALF with benign pulmonary disorder. The Th cells in BALF with lung cancer was lower than those in the benign pulmonary disorder（P〈0.05）, the Th/Ts ratio were significantly lower and the Ts cells were significantly higher in BALF of lung cancer （P〈 0.01 ） comparied with the benign pulmonary disorder; 3）The percentage of This （35.76± 7. 71）%,Tsis（64.31±7.37）% and Th/Ts are（0. 57±0. 18）in BALF with lung cancer,and the percentage of Th is （49.27±12. 88） % ,Ts is （44.54 ± 9.56）%, and Th/Ts is（1.13± 0.29）in BALF with benign pulmonary disorder. Respectively, the Th cells in BALF with lung cancer were lower than those in the benign pulmonary disorder（P〈0.05）, the Th/Ts ratio were significantly lower and the Ts cells were significantly higher in BALF of lung cancer（P〈0.01）comparied with the benign pulmonary disorder. Conclusion The immunity function in the patients with lung cancer was significantly decreased. Flow cytometric approach is valuable in analyzing T lymphocyte subsets in BALF.