人ARD1在磷状上皮及鳞状细胞癌中的表达

Expression of human ARD1 in squamous epithelium,squamous cell carcinoma

目的探讨人ARD1(hARD1)在鳞状上皮及鳞状细胞癌(SCC)中表达的意义,进一步研究hARD1的功能。方法制备anti-hARD1多克隆抗血清,以免疫组化法检测并比较hARD1在皮肤、肺、食管、宫颈的正常或良性增生、化生的鳞状上皮(对照组织),不同分化程度的SCC等组织中的表达。结果与对照组相比,hARD1在SCC中的表达降低,尤其在低分化SCC中有显著降低(P<0.001)。hARD1的表达与鳞状细胞的分化有关,相关系数r=0.493,P<0.001。结论 hARD1的表达与鳞状上皮细胞分化呈正相关,细胞分化越成熟,hARD1表达越强。

Objective To investigate the expression and possible role of human arrest defective 1（hARD1） in squamous epithelium and squamous cell carcinoma （SCC）. Methods Firstly, polyclonal anti hARD1 anlibody was prepared and characterized. Then,the expression of bARD1 in various differemialed SCC and squamous epithclium（control tissucs） which were from skins, lungs,oesophaguses and cervices was detected by immunohistochemical method and was compared with each other. Results Compared with control tissues,the expression of hARD1 was significantly down regulated in squamous cell carcinoma,especially in poor differentiated carcinoma（P〈0. 001） and was positively associaled with the differentialion of squamous cell（r=0. 493,P〈0. 001）. Conclusion The expression of hARD1 was positively associated with the differemiation of squamous cell. Namely,Poorer differentiation tended to show lower bARD1 expression.