六种梅毒初筛试验试剂的评价

Evaluation of six primarily screen reagents for Treponema pallidum

目的了解6种梅毒初筛试验试剂之间的差异。方法用6种不同的梅毒初筛试剂同时检测82例梅毒患者的血清和33例自身抗体阳性患者的血清。结果 3个厂家生产的TRUST试剂,在结果为定性、半定量都为阴性的样本中,TRUST试剂2有7例(7/82),而TRUST试剂1和3为0例,差异有统计学意义(P<0.05)。在结果为定性阳性、半定量为1:128的样本中,RPR试剂1有6例(6/82),RPR试剂2有0例,两种试剂差异有统计学意义(P<0.05)。6种试剂没有发现生物学假阳性。结论梅毒初筛试验要选择一种质量好的试剂,并同时作好性病实验室室内质控,才能确保实验结果的准确性,为临床提供可靠的实验依据。

Objective To investigate the differences between six primarily screen reagents for Treponema pallidum. Methods Using six different Treponema pallidum reagents test 82 serum from Treponema pallidum patients and 33 serum from self-antibody positive patients. Results Among three TRUST reagents,There were 7 in TRUST reagents 2 （7/82）, 1 in TRUST 1,0 positive in TRUST 3 for qualitative and self-quantitative negative samples. There were significant differences between them（x^2 = 5.53, P〈0.05）. There were six in RPR 1 （6/82）, 0 in RPR 2 for qualitative positive, self-quantitative 1 ： 128 samples, There were significant differences between them （x^2 =4.45 ,P〈0.05）. There were no biological false positive in six reagents. Conclusion We should choose one high-quality reagent for Treponema pallidum primarily screen, also we must do interior quality control in venereal disease laboratory for accuracy of measurement results.