霍奇金病的病理及免疫组织化学研究

A STUDY OF PATHOLOGY AND IMMUNOHISTO CHEMISTRY IN HODGKIN’S DISEASE

为探讨ＨＤ中Ｒｅｅｄ－Ｓｔｅｒｎｂｅｒｇ细胞的组织学起源，应用免疫组织化学方法检测４８例ＨＤ中ＣＤ３、ＣＤ２０、ＣＤ１５、ＣＤ３０和ＭＩＢ１的表达。结果显示，９例ＬＰＨＤ中６例Ｒｅｅｄ－Ｓｔｅｒｎｂｅｒｇ细胞表达ＣＤ２０，１６例ＮＳＨＤ中３例表达ＣＤ２０，所有的Ｒｅｅｄ－Ｓｔｅｒｎｂｅｒｇ细胞不表达ＣＤ３，大部分Ｒｅｅｄ－Ｓｔｅｒｎｂｅｒｇ细胞表达ＣＤ１５，所有的ＲＳ细胞表达ＣＤ３０和ＭＩＢ１。提示，ＨＤ中ＲＳ细胞可能为Ｂ细胞来源。

In order to study the origin of Reed Sternberg cells in Hodgkin's disease, an immunochemical method was used to detect the expression of CD 3、CD 20 、CD 15 、CD 30 and MIB1 in 48 cases of Hodgkin's disease. The result showed that Reed Sternberg cells in 6 of 9 cases with LPHD and in 3 of 16 cases with NSHD expressed CD 20 ; all Reed Sternberg cells did not react with CD 3;a majority of Reed Sternberg cells was positive for CD 15 ;all of Reed Sternberg cells were positive for CD 30 and MIB 1. Our result suggested that some of Reed Sternberg cells might be derived from B cells. CD 15 and CD 30 are useful antibodies for the diagnosis of Hodgkin's disease. All of Reed Sternberg cells are being a proliferating statu.