促甲状腺素释放激素与多巴胺伍用抗高原创伤低血容量性休克大鼠的作用

Effects of thyrotropin releasing hormone in combination with dopamine on traumatichemorrhagic shock in rats at high altitude

目的：观察促甲状腺素释放激素（ＴＲＨ）与多巴胺（ＤＡ）伍用对高原创伤低血容量性休克大鼠的治疗作用。方法：初进高原大鼠２８只，分生理盐水对照组（８只），ＴＲＨ组（７只），ＤＡ组（６只）和ＴＲＨ与ＤＡ合用组（７只）。大鼠右侧股骨粉碎性骨折加放血（血压６．０ｋＰａ维持１小时）复制创伤低血容量性休克模型，观察ＴＲＨ（５ｍｇ／ｋｇ）、ＤＡ（１ｍｇ／ｋｇ）和ＴＲＨ与ＤＡ半量合用对创伤低血容量性休克大鼠血流动力学指标和大鼠存活时间的影响。结果：ＴＲＨ单用或与ＤＡ合用能显著提高休克大鼠的血压，改善心肌收缩向量环面积（Ｌｏ），两药单用或合用均能改善休克大鼠左心室收缩压（ＬＶＳＰ）、左心室收缩压最大变化速率（±ｄｐ／ｄｔｍａｘ）、心肌最大收缩速度（Ｖｐｍ）；ＴＲＨ单用及与ＤＡ合用可明显延长休克大鼠的存活时间，合用效果优于ＴＲＨ或ＤＡ单用效果，并显示出一定的协同作用。结论：ＴＲＨ与ＤＡ合用抗高原创伤低血容量性休克有一定的协同作用。

Objective:To investigate the beneficial effect of thyrotropin releasing hormone(TRH) in combination with dopamine on traumatichemorrhagic shock in rats at high altitude (lasa,Tibet).Methods:Twentyeight Wistar rats were subjected to traumatichemorrhagic shock by femur fracture and hemorrhage.Rats were respectively given 5 mg/kg TRH ( n =7),1 mg/kg dopamine ( n =6),and 2 5 mg/kg TRH plus 0 5 mg/kg dopamine ( n =7).In the control group,animals received equal volume of normal saline solution.The changes in hemodynamics and survival time were observed in rats following drug administration.Results:The results showed that either TRH or dopamine could significantly improve the hemodynamic parameters including LVSP,±dp/dtmax,Vpm,and Lo,and prolong the survival time of traumatichemorrhagic shock rats.However,treatment with TRH in combination with dopamine was superior to the effect of TRH or dopamine alone.Conclusions:It is suggested that TRH and dopamine have synergistic therapeutic effects on traumatichemorrhagic shock at high altitude.