蔗糖对GK/Wistar大鼠糖脂代谢及肝胰组织的影响

Effect of sucrose on glucose and lipid metabolism and the pathological changes of liver and pancreas in GK and Wistar rats

目的研究蔗糖对GK/Wistar大鼠糖脂代谢及其肝、胰组织的影响。方法GK和Wistar大鼠各32只,随机分为W(Wistar)组、WS(Wistar+30%蔗糖)组、G(GK)组和GS(GK+30%蔗糖)组。WS、GS组给予30%蔗糖饮水,另两组给予正常饮水。实验期间每周测定一次空腹血糖,于实验开始后的2、4、6、10周时间点处死部分动物,收集血液并分离肝脏和胰腺,测定甘油三酯、葡萄糖、总胆固醇等生化指标,常规组织病理学检测肝脏和胰腺的病理改变。结果给予蔗糖水后,随着时间的延长,WS组体重和血清TG呈升高趋势,血清TC、Glu未发现相应的趋势,胰腺、肝脏病理损伤呈加重趋势,且胰腺损伤出现时间早于肝脏;GS组除WS组的发现外,血清TC呈先降低后恢复趋势,血清Glu呈先降低后升高趋势,且胰腺损伤要比WS组出现得更早、更严重,肝脏损伤也更严重。结论蔗糖可引起或加重GK/Wistar大鼠的糖脂代谢紊乱以及肝胰组织的病理性损伤,并呈现一定的时效关系。

Objective To study the effect of sucrose on glucose and lipid metabolism and the pathological changes in liver and pancreas of diabetic and normal rats.Methods 32 Wistar rats and 32 GK rats were randomly divided into W （Wistar）,WS （Wistar＋30% sucrose）,G （GK） and GS （GK＋30% sucrose ） groups.There was 30% sucrose in the drinking water for WS and GS groups and no sucrose in the drinking water for W and G groups.The metabolism of glucose and lipid and the pathological changes of liver and pancreas were observed at the 2nd,4th,6th and 10th week of the experiment respectively.Results The body weight and serum TG of WS group increased gradually after drinking water with sucrose,while serum Glu and TC were not changed.There were some pathological lesions in the liver and pancreas of WS group,and the occurrence of lesions in pancreas was earlier than that in liver.Besides what discovered in WS group,serum TC of GS group decreased initially and then gradually recovered,while serum Glu decreased initially then gradually increased.In comparison with WS group,the occurrence of pancreas and liver injuries in GS group was earlier and severer than those in WS group.Conclusion Sucrose is harmful for GK and Wistar rats,and could induce or exacerbate the disorder of glucose and lipid metabolism,the pathological lesions of liver and pancreas,and showed a time-response relationship.