复配式粗杂粮对大鼠胰岛素抵抗及PPAR-γ表达的影响

Effect of compound coarse grain food on insulin resistance and expression of PPARγ in rat

目的掌握复配式粗杂粮对高脂饮食诱导的大鼠胰岛素抵抗的影响。方法40只SD大鼠随机分为阴性对照组(n=10)和高脂造模组(n=30),分别给予基础饲料和高脂饲料,6周后再将高脂造模组分为高脂模型对照组、米面组、粗杂粮组(n=10),提供相应饲料。继续喂养9周后,测定大鼠血糖和胰岛素水平,RT-PCR法测定脂肪组织中过氧化物酶体增殖物激活受体-γ(PPAR-γ)mRNA表达。结果6周高脂饮食后,高脂造模组胰岛素抵抗指数(HOMA-IR)明显升高,造模成功。粗杂粮组大鼠的体重和HOMA-IR显著低于高脂模型对照组和米面组,粗杂粮组PPAR-γmRNA的表达与其他3组比较明显增加(P<0.05)。结论复配式粗杂粮能显著降低高脂饮食引起的胰岛素抵抗大鼠的血糖、胰岛素水平,改善胰岛素敏感性,其机制可能与增加PPAR-γ的表达有关。

Objective To explore the effect of compound coarse grain food on insulin resistance （IR） in rats.Methods Forty Sprague-Dawlay rats were randomly divided into normal control group （n=10） and model group （n=30）,provided regular diet and high fat diet separately.After 6 weeks,the model group were divided into high fat diet group,rice flour group and coarse grain group,and provided the corresponding feed.After feeding 9 weeks,the fasting blood glucose,insulin and the expression of PPAR-γ mRNA in rats were measured.Results IR was successfully induced by 6-weeks high fat diet.The body weight and insulin resistance index （HOMA-IR） in coarse grain group were significantly lower than high fat diet group and rice flour group.The expression of PPAR-γ mRNA in coarse grain group was significantly higher than other three groups （P〈0.05）.Conclusion The coarse grain food can reduce the concentration of blood glucose and insulin in rat with insulin resistance induced by high-fat dietary,and improve insulin sensitivity.The possible mechanism is the over-expression of PPAR-γ.