氟对暴露人群下丘脑-垂体-性腺轴激素水平的影响

Effect of fluoride on human hypothalamus-hypophysis-testis axis hormones

目的探讨氟对成人下丘脑-垂体-性腺轴生殖激素水平的影响。方法选择水氟浓度为3.89mg/L的开封孙营村为调查区,另选水氟浓度小于1.0mg/L的沈李楼村作为对照区。对两区居住5年以上全部居民进行健康体检,并测定尿氟含量。随机抽取空腹静脉血150份,应用放射免疫方法测定血清促性腺激素释放激素(GnRH)、促黄体生成素(LH)、睾酮(T)和雌二醇(E2)水平。结果高氟区水氟、土壤氟和粮食氟含量均高于对照区(P<0.05),且高于国家标准;空气氟两地区均未检出。高氟区人均日摄氟量高于对照区(P<0.05),且高于国家标准。高氟区人群尿氟含量明显高于对照区(P<0.05)。高氟区男性、女性血清GnRH水平与对照区比较差异均无显著性。高氟区男性血清LH水平明显高于对照区(P<0.05),女性血清LH水平与对照区比较,差异无显著性。高氟区男性血清T水平明显低于对照区(P<0.05),而女性血清T水平则明显高于对照区(P<0.05)。高氟区男性、女性血清E2水平与对照区比较差异均无显著性。结论氟能影响成人下丘脑-垂体-性腺轴各层面生殖激素水平,因而表现出生殖内分泌干扰作用,且对男性的影响大于女性。

Objective To study of endocrine disturbing effect of fluoride on human hypothalamus-hypophysis-testis axis hormones.Methods Sunying County,Kaifeng City was selected as polluted district which the fluoride in drinking water was 3.89 mg/L,and Shenlilou county was selected as control district which the fluoride was less than 1.0 mg/L.150 individual lived there more than 5 years were selected randomly.And investigated by medical examination,then blood and urine sample were collected,and the serum level of gonadotropin-releasing hormone（GnRH）,luteinizing hormone（LH）,testosterone（T） and estradiol（E2） were measured by RIA method,and the urine level of fluoride were measured.Other than that,the concentration of fluoride in the water,food,soil and air were detected by the standard methods.Results The concentrations of fluoride in the water,food and soil of the fluoride polluted district were significantly higher than those of control district （P〈0.05）,and the concentration fluoride in the air of two district were not found.There was no significant difference of serum level of GnRH between fluoride polluted district and control district （P〉0.05）.The serum level of LH in men of fluoride polluted district was significantly higher than that of control group （P〈0.05）,and the serum level of T in men of fluoride polluted district was significantly less than that of control group （P〈0.05）.There was no significant difference of serum level of LH between fluoride polluted district and control district （P〉0.05）,and the serum level of T in women of fluoride polluted district was significantly higher than that of control group （P〈0.05）.There was no significant difference of serum level of E2 between fluoride polluted district and control district （P〉0.05）.Conclusion Fluoride could effect hormone levels of each layer of the hypothalamus-hypophysis-testis axis,and show the reproductive endocrine disturbing effects.The reproductive endocrine disturbing effects of male maybe more severe than those of female.