烟蚜羧酸酯酶和乙酰胆碱酯酶的体躯分布特征

Distribution Characteristics of Carboxylesterase and Acetylcholinesterase in Body of Myzus persicae

为了探明烟蚜体躯的酶活性分布特征,对烟蚜羧酸酯酶(CarE)和乙酰胆碱酯酶(AChE)的体躯分布进行了研究。结果表明:成虫的头、胸、腹羧酸酯酶的活性分别为19.80%、40.14%和40.78%;3龄若蚜的头、胸、腹羧酸酯酶的活性分别为13.50%、44.50%和42.00%;4龄若蚜的羧酸酯酶的活性分别为17.51%、41.67%和40.82%。成虫、3龄若蚜和4龄若蚜均以头部的活性最低,并且均极显著低于胸部和腹部(p<0.01)。成虫、3龄若蚜和4龄若蚜的头部乙酰胆碱酯酶的活性分别占总活性的32.09%、32.00%和32.05%,并且头部活性显著低于胸部和腹部的活性(p<0.05)。

The distribution of CarE and AChE in body of Myzus persicae was studied to probe the enzyme activity distribution of two tested enzymes in body of Myzus persicae. The results showed that the CarE activity in head, thorax and abdomen of adult Myzus persicae were 19.80%, 40.14% and 40.78%, the CarE activity in head, thorax and abdomen of Myzus persicae nymph with the third instar were 13. 500%, 44.50% and 42.00% and the CarE activity in head, thorax and abdomen of Myzus persicae nymph with the fourth instar was 17.51%, 41.67% and 40. 820% respectively. The CarE activity in head of Myzus persicae adult, nymph with the third instar and nymph with the fourth instar was the lowest and was very significant lower than that in thorax and abdomen of Myzus persicae adult, nymph with the third instar and nymph with the fourth instar. The AChE activity in head of Myzus persicae adult, nymph with the third instar and nymph with the fourth instar was 32.09%, 32.00% and 32.05% respectively and was significant lower than that in thorax and abdomen of Myzus persicae adult, nymph with the third instar and nymph with the fourth instar.