H_2O_2和NO对滑膜成纤维细胞增殖反应的影响

Effects of H2O2 and NO on the Proliferation of Synovial Fibroblast

采用＾３Ｈ－ＴｄＲ掺入法和ＭＴＴ比色法研究过氧化氢（Ｈ２Ｏ２）和一氧化氮（ＮＯ）对兔滑膜成纤维细胞（ＳＦ）增殖反应的影响。结果表明，在使用亚适ＳＦ浓度（５×１０＾７细胞／Ｌ）时，＾３Ｈ－ＴｄＲ掺入法和ＭＴＴ比色法检测结果一致，Ｈ２Ｏ２和亚硝基铁氰化钠（ＮＯ供体）在低浓度时促进ＳＦ增殖反应，而在高浓度时抑制其增殖反应，提示Ｈ２Ｏ２和ＮＯ对ＳＦ增殖反应具有双向调节作用。

Effects of H2O2 and NO on the proliferation of synovial fibroblasts (SF) were studied by using 3H-TdR incorporation and MTT colorimetric assay. The results showed that the proliferation of rabbit SF measured by 3H-TdR incorporation assay was consistent with that by MTT colorimetric assay when suboptimal concentration (5×10~7 cells/L) of SF was used. Both H2O2 and sodium nitroprusside (NO donor) could enhance SF proliferation in lower concentration and suppress its proliferation in higher concentration, suggesting that H2O2 and No possess two-sided regulatory effects on SF proliferation.