慢性HCV感染状态与机体细胞免疫功能关系的探讨

Study on the Relationship between Immune Function and the Chronic HCV Infection

目的:探讨慢性丙型肝炎病毒(HCV)感染患者丙型肝炎病毒RNA(HCV RNA)增殖状态与T细胞亚群功能及血浆中IL-2和slL-2R活性的关系。方法:以间接免疫荧光法,ELISA法和RT-PCR分别检测75名慢性HCV感染患者(其中HCV RNA阳性47例,HCV RNA阴性28例)外周血T细胞亚群,IL-2及sIL-2R的水平和HCV RNA。结果:慢性HCV感染患者周围血CD3^+,CD4^+淋巴细胞亚群,CD4^+/CD8^+比值及IL-2水平均显著低于正常对照组(P<0.01),而sIL-2R明显升高(P<0.01);血清HCV RNA阳性患者T细胞亚群,CD4^+/CD8^+比值及IL-2水平显著低于HCV RNA阴性患者(p<0.01)。结论:慢性HCV感染患者的机体免疫功能紊乱。细胞免疫功能低下,HCV RNA阳性患者较阴性患者更甚,提示细胞免疫功能受抑可能是HCV持续增殖的原因。

To investigate the relationship between cellular immune function and the infection status of viruses in chronic HCV infection,peripheral blood T-lymphocyte subsets and serum levels of IL-2 and sIL-2R were determined by indirect immuno-fluorescence and ELISA in 75 patients with chronic HCV infection, of which 45 cases were HCV-RNA positive and 28 cases HCV-RNA negative. The HCV-RNA was determined by RT-PCR. It was found that the CD3+ and CD4+ T cell subsets , CD4+/CD8+ ratio and the blood levels of IL-2 of patients were significantly lower than those of the controls, but the level of sIL-2 increased significantly. The T cell subsets, CD4+/CD8+ ratio and blood levels of IL-2 of HCV-RNA positive cases were significantly lower than those of the HCV-RNA negative cases. It suggests that suppression of the cell mediated immunity might be the cause of HCV persistant infection.