摘要
对2005年,2007年和2009年产下关生沱茶,进行HT-29结肠癌细胞体外抗癌效果评价,通过MTT试验、细胞生长率试验和RT-PCR分析验证其抗癌效果。200μg/mL质量浓度下2005年产下关生沱茶(85%)表现出对HT-29结肠癌细胞最强的生长抑制效果。RT-PCR检查Bax,Bcl-2基因表达情况显示2005年产下关生沱茶对HT-29结肠癌细胞有最强的诱其凋亡的能力。由此得出,储存时间越长的沱茶具有更好的抗癌预防效果。
The Xiaguan Raw Bowl Tea manufactured in 2005, 2007 and 2009 were evaluated for their in vitro anticancer effect on HT-29 human colon cancer cells. MTT assay, growth inhibition tests and RT-PCR were employed to check anticancer effect. At the concentration of 200μg / ml, Raw Bowl Tea manufactured in 2005 （85%） exhibited the highest inhibitory effects on the growth of HT-29 colon cancer cells. By bax and bcl-2 expression in mRNA, Raw Bowl Tea manufactured in 2005 showed the highest induced apoptosis effect. The results suggest that the longer the storage time, the more anticancer effect the Raw Bowl Tea has.
出处
《食品工程》
2010年第1期37-39,共3页
Food Engineering
