HPLC法测定尼麦角林原料药的含量

Optimization of HPLC Condition for Determination of Nicergoline

目的:优化HPLC法测定尼麦角林原料药的含量的色谱条件。方法:以十八烷基硅烷键合硅胶为填充剂,磷酸缓冲液(取磷酸二氢钾6.8g,加水溶解并稀释至1000ml,用三乙胺调节pH为7.0)-甲醇-乙腈(30:35:35)为流动相,检测波长为288nm。结果:尼麦角林在10-500mg线性关系良好,相关系数r=0.9997,回收率在99.0%-101.5%之间,RSD(%)均小于1%。结论:该法可靠性高,准确性和重复性好,适合于该产品的质量检验分析。

Objective： To optimize HPLC condition for determination of Nicergoline. Methods： This analysis was performed on octadecylsilane chemically bonded silica, with a mobile phase of Phosphate buffer solution （taking potassium dihydrogen phosphate 6.8g, dissolved in water and diluted to 1000ml, adjusted pH 7.0 with triethy]amine） -methanol - aceto- nitrile （30：35：35 ）. The detection wavelength was set at 288 ran. Results ：The linearity was obtained over 10 -500rng and the linear relation was good （r = 0.9995 ）. The recovery was 99.0% -101.5% ,and RSD （%）were less than 1%. Condusion：This method is reliable, accurate and repeatable. It can be used for quality control of Nicergoline.