野桑蚕谷胱甘肽-S-转移酶(GSTs)基因的诱导表达定量分析

Quantitative analysis of induced expression of GST genes in the wild mulberry silkworm, Bombyx mandarina

谷胱甘肽-S-转移酶(GSTs)在机体的解毒代谢和抗氧化中起重要作用。为了从分子水平上探究野桑蚕Bombyx mandarina对杀虫剂抗性的产生机理,本研究采用实时荧光定量RT-PCR方法,对GSTs基因在正常饲养野桑蚕5龄幼虫及用敌敌畏和溴氰菊酯处理5龄幼虫不同组织中的转录水平进行检测,并采用Actin3内源参照基因对检测结果进行归一化处理。结果表明:用敌敌畏和溴氰菊酯处理5龄幼虫24h后,GSTs基因在各组织中的诱导转录水平存在差异,但GSTs基因在脂肪体中的诱导转录水平最高,其次为中肠,可能与这2种组织是野桑蚕主要的解毒器官有关。其中,GSTe2和GSTe5基因诱导转录水平相对较高,推测这2个基因可能主要参与野桑蚕对外源物质的解毒代谢。

Glutathione S-transferases （GSTs） play an important role in detoxification and antioxidation. In order to study the molecular mechanisms of insecticide resistance in the wild mulberry silkworm, Bombyx mandarina, we detected the transcription levels of GST genes in different tissues of the fifth instar larvae and those treated with dichlorovos and dehamethrin using real-time quantitative RT-PCR method, and took Actin3 gene as reference to dispose the results. The results showed that the expression levels of GST genes induced by dichlorovos and deltamethrin were different in various tissues, the highest expression level was found in fat body, and the second was in the midgut, and this might be due to that both tissues are major organs involved in detoxication of the pesticides. Furthermore, the GSTe2 and GSTe5 genes were highly expressed, so it is inferred that these genes may be mainly responsible for detoxification of xenobiotics.