摘要
目的分析下调bcl11b基因表达对人T淋巴细胞白血病细胞株Molt-4细胞增殖和凋亡的影响。方法通过核转技术将bcl11b-siRNA转染Molt-4细胞;在不同时间点分别采用荧光实时定量PCR观察其对bcl11b基因表达的影响;MTT法观察对Molt-4细胞体外增殖的抑制作用;流式细胞术及刘氏染色检测siRNA的诱导凋亡作用。结果转染后24小时开始细胞生存率均显著降低(P<0.01),细胞发生凋亡,bcl11b基因表达下调。结论bcl11b-siRNA可抑制Molt-4细胞中bcl11b基因表达,使肿瘤细胞增殖抑制和发生凋亡。
Objective To investigate the effects of down-regulating B-cell chronic lymphocytic leukemia / lymphoma 11B gene expression on the proliferation and apoptosis of Moh-4 cells. Methods The small in terfering RNAs targeted on bel11b gene were transfected into Molt-4 cells by nucleofeetor techique. The levels of bel11b mRNA expression were analyzed at different time points after nucleofection by Real-time quantitative PCR. The cells proliferation rate was evaluated by MTT assay. Cell aapoptosis was detected by flow cytometry and modified Wright staining. Results The survival rates of siRNA-transfected ceils had significantly decreased since the 24 hours after transfection (P〈0. 01). Apoptosis of treated molt-4 ceils could be identified. The level of bcl11b mRNA expression was decreased. Conchtsion bcl11b gene expression was inhibited by RNA interference selectively. It might inhibit the proliferation and induced the apoptosis of leukemia T cells.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第3期247-250,共4页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目(30771980)
广东科技计划资助项目(2007B030703008
2009B050700029)
血液病学国务院侨办重点专科基金资助项目(51205002)