双氢青蒿素诱导前列腺癌PC-3细胞凋亡

Apoptosos of Human Prostate Cancer Cell Line PC-3 Induced by Dihydroartemisinin

目的研究双氢青蒿素(DHA)诱导前列腺癌PC-3细胞凋亡作用,并观察其形态学变化。方法采用人前列腺癌PC-3细胞建立裸鼠种植瘤模型,20只模型鼠随机分为对照组、溶剂组、DHA大剂量组及小剂量组,经13天干预后,计算抑瘤率;HE染色观察肿瘤组织形态学表现;TUNEL法及Hoechst33258荧光染色检测凋亡。结果DHA大小剂量组抑瘤率分别为69.221%和63.186%;肿瘤组织内凋亡细胞明显增多;肿瘤细胞凋亡率及凋亡细胞数密度均明显升高(P<0.05)。结论DHA具有较强的抗肿瘤作用,其作用机制可能与诱导肿瘤细胞凋亡有关。

Objective To study the effects of dihydroartemisinin （DHA） on inducing apoptosis of human prostate cancer PC-3 cells and investigates the morphological changes. Methods Prostate cancer PC-3 cells were transplanted into 20 nude mice to establish the solid tumor mode. These nude mice were randomly divided into 4 groups with 5 mice in each group： control group , solvent group, low dose DHA group and large dose DHA group. The tumor growth inhibition rates were calculated on day 13 after drug administration. Pathomorphism changes of PC-3 cells were observed by light microscope （LM） after administration of DHA. Apoptosis cells were detected by HE staining,TUNEL method and Hoeehst 33258 fluorescence staining. Results The tumor growth inhibition rates of large close DHA group and low dose DHA group were 69. 221% and 63. 186%, respectively. LM revealed that the scattered apoptotic cells were observed in tumor tissues of DHA groups. TUNEL staining evealed that the rate of cell apoptosis raised significantly（P〈0. 05）. Fluorescence microscope examination indicated that the apoptotic cells stained by Hoechst 33258 significantly increased in tumor tissues of DHA groups. The density of apoptotic cell significantly augmented （P〈0. 05）. Conclusion These results demonstrated that DHA had stronger inhibitory effects on human prostate cancer cell line PC-3 cells in vivo. The action mechanism might be related to DHA inducing the apoptosis of prostate cancer PC-3 cell.