PTEN、NFκB、Ki-67在胃癌中的检测及意义

Detection and Significance of PTEN,NFκB and Ki-67 in Gastric Cancer

目的探讨PTEN、NFκB、Ki-67的表达与胃癌的关系及作用。方法运用免疫组织化学法检测PTEN、NFκB、Ki-67在胃癌中的表达情况。结果PTEN蛋白在胃癌组织中的阳性表达率为24.2%(15/62),明显低于癌旁正常胃组织为77.3%(17/22)(P<0.001);而NFκB、Ki-67蛋白在胃癌中的阳性表达率分别为61.3%(38/62),67.7%(42/62),明显高于癌旁正常胃组织的18.2%(4/22)和13.6%(3/22)(P<0.01,P<0.001)。PTEN、NFκB、Ki-67的阳性表达率与临床病理分期、组织分化程度及5年生存期有关,而与性别、年龄无关(P>0.05);临床病理分期低、组织分化好的患者生存时间往往较长,差异有统计学意义(P<0.05)。此外,胃癌中PTEN与NFκB呈负相关(χ2=6.519,P<0.05),PTEN与Ki-67呈负相关(χ2=4.022,P<0.05),NFκB则与Ki-67呈正相关(χ2=5.641,P<0.05)。结论PTEN基因的失活、NFκB基因的活化可能促进了胃癌的发生、发展和浸润转移的进程;Ki-67基因的表达情况不仅能客观反眏胃癌的增殖活性,还能估计肿瘤细胞发生转移的潜在危险性,可作为临床评价肿瘤恶性度及预后的指标。

Objective To investigate the relationship and action among the expressions of PTEN, NFκB and Ki-67 and gastric cancer. Methods The immunochemistry method was used to detect expressions of PTEN,NFκB and Ki-67 protein. Results The positive rates of PTEN protein expressions in gastric cancer tissue were 24. 2% （15/62）, obvious lower than those in par-cancer tissue, 77. 3% （17/22） （P〈 0. 001 ）. However, the positive rates of NFκB and Ki-67 protein expressions in gastric cancer tissue were 61.3%（38/62）and 67. 7% （42/62）, respectively, obvious lower than those in par-cancer tissue, 18.2% （4/22）and 13.6% （3/22）（P〈0. 01, P〈0. 001 ）. The positive rates of PTEN, NFκB and Ki-67 protein expressions were relation to clinical pathology stage, differentiation degree of tissue and survival periods of five years, but were independent of sex and ages（P〉0. 05） ; The patient that had lower clinical pathology stage and higher differentiation degree of tissue would had attained longer survival periods, the differences were significant（P〈0.05）. Moreover, in gastric cancer tissue, PTEN and NFκB manifested negative relationship（χ^2 = 6. 519,P〈0. 05）; PTEN and Ki-67 manifested negative relationship （χ^2 =4. 022,P〈0. 05） ,too. On the contrary , NFκB and Ki-67 manifested positive relationship（χ^2 = 5. 641 ,P 〈0.05）. Conclusion Missing actives of PTEN gene and NFκB gene activation maybe promoted a progress of origin, growth, soakage and metastasis of gastric cancer. Expressions of Ki-67 gene not only impersonally reflected multiplication activation of gastric cancer, but also estimated latency fatalness of tumour cell occurring metastasis,which could be used as a marker of clinic to appraise tumour＇s danger degree and prognosis.