p57^(kip2)与cyclin D1在上皮性卵巢癌中的表达及临床意义

Expressions of p57^(kip2) and cyclin D1 Proteins in Epithelial Ovarian Carcinoma and Their Clinical Significance

目的研究p57kip2与细胞周期素D1在上皮性卵巢癌中的表达与临床病理特征的关系,探讨其在上皮性卵巢癌发生发展过程中的作用。方法采用免疫组织化学SP法检测p57kip2与cyclinD1在41例上皮性卵巢癌,12例交界性肿瘤,24例良性肿瘤与12例正常卵巢组织中的表达。结果p57kip2和cy-clinD1在正常卵巢、良性、交界性、恶性肿瘤组织中的阳性率分别为91.7%(11/12)、79.2%(19/24)、58.3%(7/12)、46.3%(19/41)和8.3%(1/12)、29.2%(7/24)、50%(6/12)、75.6%(31/41),其中正常组与恶性组之间,良性与恶性组之间差异有统计学意义(P<0.05)。在卵巢癌中p57kip2和cyclinD1的表达与肿瘤分化程度,临床分期和有无淋巴结转移有关,且两者的表达呈负相关(r=-0.311,P<0.05)。结论p57kip2和cyclinD1可能共同参与了上皮性卵巢肿瘤的发生与发展,p57kip2的低表达甚至缺失和cyclinD1高表达对预测卵巢肿瘤的恶性程度及预后有指导意义。

Objeetive To investigate the relationship of p57^kip2 and cyclin D1 expressions with clinicopathological features of epithelial ovarian tumor. Methods SP immunohistochemical technique was applied to detect the expressions of p57^kip2 and cyclin D1 proteins in tissues of 41 patients with malignant epithelial ovarian cancer, 12 with borderline tumor, 24 with benign tumor and 12 normal ovaries. Results The positive-expression of p57^kip2 and cyelin D1 was 91.7%（11/12）, 79. 2%（19/24）, 58.3%（7/12）, 46. 3% （19/41） and 8. 3%（1/12）, 29. 2% （7/24）, 50% （6/12）, 75.6% （31/41）, respectively in normal, benign, borderline, and malignant tissues. There were significant differences between p57^kip2 and cyclin DI proteins in normal group compared with the malignant, and the benign compared with the malignant. In malignant ovarian tumors, overexpression of eyclin D1 and low-expression of p57^kip2 are significantly associated with high tumor grade, advanced clinical stage, and lymph node metastasis. Expression of cyclin D1 was negatively correlated with expression of p57^kip2. Conclusion p57^kip2 and cyclin D1 played important roles in occurrence and development of epithelial ovarian cancer. Overexpression of eyclin D1 and low-expression of p57^kip2 provide important implications of malignancy and prognosis for epithelial ovarian carcinoma.