细胞外基质金属蛋白酶诱导因子CD147和基质金属蛋白酶9在肝癌中的表达及其临床意义

Expression of extracellular matrix metalloproteinase inducer CD147 and MMP-9 in hepatocellular carcinoma and its clinical significance

目的:通过对细胞外基质金属蛋白酶诱导因子(extracellular matrix metalloproteinase inducer,EMMPRIN)CD147和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在肝癌中表达的研究,探讨CD147和MMP-9与肝癌细胞浸润和转移以及预后之间的关系。方法:采用免疫组织化学法检测CD147和MMP-9在83例肝细胞肝癌标本及15例正常肝脏组织标本中的表达。结果:MMP-9和CD147在肝癌组织中的阳性表达率分别为73.5%和68.7%,高于正常组织中的表达率6.7%和0%,经比较差异均有统计学意义(P<0.05)。CD147和MMP-9的表达与肝癌的肿块大小、TNM分期、肿瘤分化程度、门脉癌栓及术后复发呈正相关(P<0.05),而与患者的年龄、性别、甲胎蛋白(alpha fetal protein,AFP)的表达水平及Child-Pugh分级无相关性。CD147和MMP-9表达为阳性患者的生存期明显低于表达为阴性患者的生存期。CD147在肝癌中的表达与MMp-9具有显著的相关性。结论:MMP-9和CD147在肝癌组织中的表达与肿瘤的大小、TNM分期、分化程度、门脉癌栓及术后复发呈正相关,通过检测它们的表达将有助于综合判断肝癌的预后。

Objective：To study the expression of extracellular matrix metalloproteinase（MMP-9） inducer CD147 and MMP-9 protein in hepatocellular carcinoma（HCC） and explore their correlation with invasion,metastasis,and prognosis of HCC.Methods：SP immunohistochemistry was used to evaluate the expression of CD147 and MMP-9 in 83 HCC tissues and 15 normal liver tissues.Results：The positive rate was 73.5% for MMP-9 and 68.7% for CD147,significantly higher than normal tissues（MMP-9： 6.7%;CD147： 0%,P〈0.05）.Expressions of CD147 and MMP-9 were positively correlated with the tumor size,pTNM stages,differentiation degree,the portal vein cancer embolism,and postoperative recurrence（P〈0.05）,but not with the patient＇s age,gender,the serum level of alpha-fetal protein（AFP）,and the Child-Pugh grades.Patients with positive expression of CD147 and MMP-9 had shorter survival time than those with negative expression of CD147 and MMP-9.Expression of CD147 was positively related with expression of MMP-9 in HCC tissues.Conclusion：CD147 and MMP-9 had positive correlation with tumor size,pTNM stages,differentiation degree,portal vein cancer embolism,and postoperative recurrence.Detection of the expression of CD147 and MMP-9 may be helpful to make a comprehensive prediction of the prognosis of HCC.