CREM基因与特发性无精症的关系

The relationship between the CREM gene and the idiopathic azoospermia

目的探讨cAMP反应元件调节物(CREM)基因与男性不育症中特发性生精障碍的关系。方法收集特发性无精症患者外周血20例,收集具有正常生育能力志愿者外周血20例作为对照,采用PCR-SSCP银染技术对特发性生精障碍患者外周血中CREM基因进行检测;对CREM基因异常无精症睾丸穿刺组织行组织学结构观察。结果在20例无精症患者中13例出现CREM基因异常,睾丸穿刺组织结构有与"唯支持细胞综合征(生精小管上皮只有支持细胞)"相似的表型,也有精子发生停滞于不同阶段生精细胞的表型。结论CREM基因异常可能与特发性生精障碍的发病有关。

Objective To study the relationship between the cyclic AMP-responsive element modulator（CREM） gene and the obstacle of idiopathic spermatogenesis of male infertility.Methods The peripheral blood of 20 patients with idiopathic azoospermia and of 20 volunteers who have the normal fertility was collected.The CREM gene was analyzed with PCR amplification and single-strand conformation polymorphism.The testicular biopsy structure of abnormal CREM gene patients was observed.Results The CREM gene mutation was observed in 13 cases of the patients with azoospermia.The testicular histological structure of the patients with azoospermia was similar to the phenotype of＂Only sertoli cells syndrome＂,or similar to the phenotype of spermatogenesis frozen at different stages of spermatogenic cells.Conclusion The mutation of CREM gene might be related to the obstacle of idiopathic spermatogenesis.