嗅球蛋白1在肺癌组织中的表达及可能意义

Expression of OLFM1 protein in lung cancer tissue and its significance

目的通过对比嗅球蛋白1(OLFM1)基因及其蛋白在肺癌组织及其配对的正常肺组织中的表达情况,探讨其表达与肺癌发生的相互关系。方法分别采用半定量RT-PCR法和荧光实时定量PCR检测21例肺癌患者的原位肺癌组织及其配对正常肺组织中OLFM1基因的相对表达变化和相对表达量,应用免疫组织化学染色观察OLFM1蛋白在组织中的表达。结果OLFM1基因在8例肺鳞癌原发灶中的表达相对于其配对正常组织中的表达显著下调约2.2倍(P=0.028);OLFM1蛋白定位于肺腺癌细胞胞质,仅在肺腺癌表达(P<0.001),其表达在不同年龄、性别、肿瘤分期、分化程度间差异均无统计学意义(P>0.05)。结论OLFM1基因肺鳞癌中表达下调,可能是一个潜在的分子标志;而OLFM1蛋白仅在肺腺癌表达,在肺鳞癌和正常组织中均不表达,这提示不同病理组织类型肺癌的发生可能有不同的发生机制和途径。

Objective To explore the relationship of OLFM1 expression with the development of lung cancer by comparing the OLFM1 expression in lung cancer specimens and matched normal lung specimens.Methods The OLFM1 mRNA expression was examined by semi-quantitative RT-PCR and fluorescence quantitative PCR in 21 lung cancer tissues and the paired normal lung tissues,and the protein expression of OLFM1 was detected by immunohistochemistry.Results The OLFM mRNA expression levels in 8 primary lung squamous cell carcinoma tissues were decreased by 2.2 folds compared with that in the matched normal lung tissues,with the difference being significantly different（P=0.028）.The OLFM1 protein was located in cytoplasm and only expressed in lung adenocarcinoma tissues（P0.001）,and the expression was not associated with patients＇ age,gender,tumor stage,and differentiation degrees（P0.05）.Conclusion The lower expression of OLFM1 gene in lung squamous cell carcinoma may serve as a potential molecule marker for the condition.The expression of OLFM1 protein in lung adenocarcinoma,but not in lung squamous cell carcinoma and normal lung tissue,may suggest that different pathological types of lung cancers may have different pathological mechanisms.