HPLC-TOFMS法对白花蛇舌草药材及其注射剂中化学成分的快速分离与鉴别

HPLC-TOFMS in rapid separation and identification of chemical components in Oldenlandia diffusa and its injection preparations

目的采用HPLC-TOFMS对白花蛇舌草药材及其注射剂中化学成分进行快速分离鉴别。方法色谱柱Ag-ilent Zorbax SB-C18柱(4.6mm×250mm,5μm),以0.3%醋酸(A)-甲醇(B)为流动相,梯度洗脱,0～30min,体积分数30%～90%B,进样量10μl,流速1.0ml/min,柱温为30℃,飞行时间质谱配有ESI离子源,质量数扫描范围m/z100～1000。结果采用HPLC-TOFMS方法对白花蛇舌草药材及其注射液制剂进行了在线化学成分分离与质谱表征,一次性在一张图谱上共鉴别出白花蛇舌草中11个化学成分,市售注射剂中6个成分,自制注射剂中2个成分。结论这可为中药白花蛇舌草及其注射剂的药效物质基础和质量控制研究奠定基础。

Objective To rapidly separate and identify the chemical components in traditional Chinese herbal medicine of Oldenlandia diffusa and its injection preparations by high performance liquid chromatography-time of flight mass spectrometry（HPLC-TOFMS）.Methods An Agilent Zorbax XDB-Cl8 column（250 mm×4.6 mm,5 μm） was used for separation and identification of chemical components in Oldenlandia diffusa,with a mobile phase of 0.3% acetic acid（A） and methanol（B） in gradient elution,0-30 min,30%-90%B.The flow rate was set at 1.0 ml/min and the injection volume was 10 μl.The time-of-flight mass spectrometer was equipped with an EIS ion source.The scanning mass range was between m/z 100-1 000.Results The traditional Chinese medicine of Oldenlandia diffusa and its injection preparation were on-line separated and characterized by HPLC-TOFMS,and 11 chemical compounds were identified in Oldenlandia diffusa,6 compounds in market injection preparation,and 2 compounds in the injection prepared by ourselves.Conclusion Chromatographic demonstration of chemical compounds in Oldenlandia diffusa in one run provides a foundation for the further studying the metabolism and mechanism of Oldenlandia diffusa and its injection preparations.