通关藤中甾体化合物的电喷雾质谱裂解规律研究

Fragmentation Pathways of Steroids from the Stems of Marsdenia tenacissima by ESI-MS/MS

利用电喷雾串联质谱(ESI-MSn)研究从云南产通关藤(Marsdeniatenacissi ma(Roxb.)Wight et Arn)中分离纯化得到的19种甾体化合物的裂解规律,分析了它们从分子离子出发的多级质谱的裂解碎片,主要报道了tenacigenoside B,tenacigenoside G,tenacigenoside H在ESI正离子模式下[M+Na]+的裂解途径和碎片离子峰,结合其他16种化合物的裂解实验,说明这些甾体化合物主要丢失C-11和C-12位上的取代基,其次是糖苷上糖顺序断裂。这有助于依据此类化合物结构特点和裂解规律来推断其结构,为此类化合物的快速鉴定提供依据。

Total of 19 steroids fragmentation pathways were investigated by electrospray ionization tandem mass spectrometry （ESI-MS^n） . Those steroids were isolated from the stems of Marsdenia tenacissirna, which collected from Yun Nan province. And the fragmentation pathways of tenacigenoside G, tenacigenoside B, tenacigenoside H were summarized detailly in positive mode[M＋Na]^＋. Combining with the experiment of other 16 compounds, the data reveals the first loss position is C-11 and C-12, then glycosides of C-3 lose orderly. This helps deduce the structures of those compounds according their structural characteristics and loss fragmentations. It also provides the basis for rapid identification of such compounds.