摘要
高温是小麦产量提高的一个重要限制因素,小麦受到高温胁迫时会产生一系列热激蛋白来适应这种逆境,不同发育阶段的热激蛋白存在差异,不同抗热作物品种中热激蛋白也存在差异,因此,研究抗热性不同的小麦品种中热激蛋白差异对小麦耐热性的研究具有重要的理论意义与实践意义。该实验利用SDS-PAGE电泳技术对2种不同的小麦品系在34.37、40℃下处理1~7h产生的热激蛋白进行研究,发现其可溶性蛋白电泳图谱与常温下的有区别,其中新蛋白的出现说明高温能够诱导新蛋白。71321-9中发现的3种新蛋白的分子量分别为97.4kD、49kD、37kD,另外还发现3种蛋白质含量明显升高,这3种蛋白质的分子量范围在17~66kD。71321-4小麦中发现了2种新增热激蛋白(37kD和29kD)及1种蛋白质的含量明显增加。这些蛋白可能与小麦幼苗抗热性有关,其含量的增加能够提高幼苗的抗热性。
High temperature is an important limiting factor to the improvement of wheat yield. When suffered hot stress,wheat will produce a series of heat shock proteins to adapt this adversity. In different upgrowth phases,or different heat-resistant crop varieties,the heat shock protein exist differences. Therefore,study on differences of heat shock protein has significant theoretical and practical meaning for researching heat resistance of wheat. By using SDS-PAGE method,the inducing conditions and manifestation of heat shock protein were studied,which provided theoretical basis for yield increasing of wheat.
关键词
热激蛋白
小麦
品种
电泳
Heat shock protein
Wheat
Variety
Electrophoresis