摘要
[目的]研究苦荞醇提物的镇静催眠作用,为进一步扩大苦荞临床应用奠定基础。[方法]将颗粒饱满的苦荞带壳种子干燥后适度研碎,75%乙醇(固液比1∶10),85℃回流提取,提取2次,每次2h,合并2次滤液,回收乙醇并浓缩至稠膏状,经80℃烘干,得苦荞提取物,提取物得率为6.67%。记录苦荞醇提物对戊巴比妥钠引起的小鼠睡眠持续时间、入睡率及小鼠自主活动次数。将40只雄性小鼠随机分为4组,每组10只,分别为空白对照组、苦荞醇提物高剂量组、苦荞醇提物低剂量组和阳性药物对照组;试验前8h禁食不禁水,试验时空白对照组给予等体积的0.5%CMC-Na,苦荞低剂量组、高剂量组分别给0.13g/kg、0.5g/kg苦荞醇提物,阳性药物对照组给2.28g/kg的补脑安神片;连续给药7d。①末次给药30min后,小鼠腹腔注射戊巴比妥钠45mg/kg(由预试验确定),记录睡眠出现和消失的时间,计算睡眠潜伏期(睡眠出现时间-记录开始时间),睡眠持续时间(睡眠消失时间-睡眠出现时间);②末次给药后30min,小鼠腹腔注射戊巴比妥钠35mg/kg(戊巴比妥钠阈下催眠量,即90%～100%小鼠不入睡的戊巴比妥钠最大剂量,由预试验确定),观察并记录入睡动物数(注射后15min内小鼠翻正反射消失达1min以上者记为入睡),计算各组入睡率;③末次给药30min后将小鼠放入小鼠自主活动测定仪中,让其适应5min,然后开始测定5min内自主活动次数。[结果]苦荞醇提物能延长戊巴比妥钠阈上剂量引起的小鼠睡眠持续时间,增加戊巴比妥钠阈下剂量引起的小鼠睡眠只数,且能明显减少小鼠自主活动次数。[结论]苦荞醇提物具有镇静作用。
[Objective]The aim was to study the sedative and hypnotic effects of Tartary Buckwheat ethanol extract and lay a foundation for further expanding the application of Tartary Buckwheat. [Method]The effects of Tartary Buckwheat ethanol extract on pentobarbital sodium and mice spontaneous action times were recorded. [Result]Ethanol extract of Tartary Buckwheat could prolong mice sleep duration caused by pentobarbital sodium （suprathreshold dosage）,increase mice sleep number caused by pentobarbital sodium （subthreshold dosage）,and obviously inhibit mice spontaneous action times. [Conclusion]Ethanol extract of Tartary Buckwheat has a function of sedation.
基金
Supported by Key Projects in the National Science &Technology Pillar Program during the Eleventh Five-year Plan Period(2006BAD02B06)
Projects in Sichuan Province Administration of Traditional Chinese Medicine (2006750)~~
关键词
苦荞醇提物
镇静
催眠
小鼠
Ethanol extract of Tartary Buckwheat
Sedation
Hypnosis
Mice
