摘要
[目的]分析乌拉尔甘草中6种微量元素的初级形态,为阐明甘草药效原理和探讨人工种植甘草质量控制提供理论依据。[方法]按照传统煎煮法对人工种植乌拉尔甘草中Cu、Zn、Fe、Ca、Mg、Mn6种元素进行提取;用微孔滤膜分离提取液中的可溶态与悬浮态;采用火焰原子吸收光谱法对可溶态中的6种元素进行测定。[结果]Ca含量最高,为3.572mg/g,其次为Mg、Fe、Zn、Mn、Cu含量最低,为3.81mg/kg。甘草中6种微量元素的含量随元素种类不同而有显著差异。甘草中6种元素的总提取率为1.71%~60.06%,浸留比为0.0183%~1.6820%。其中,Zn的提取率最高,为60.06%;其次为Mn、Cu和Ca,分别为51.86%、22.26%和19.38%,表明Zn和Mn元素的存在形态较易溶于水,与甘草具有镇痛、提高免疫功能等作用可能存在密切的关系。Zn的浸留比最大为1.68,是甘草中作用最大的元素或最特征的元素。火焰原子吸收光谱法对各元素的加标回收率为95.72%~103.15%,相对标准偏差小于2.38%。[结论]火焰原子吸收光谱法准确性高,用以分析乌拉尔甘草中微量元素的初级形态是可行的。
[Objective]The aim was to analyze the primary speciation of 6 microelements in Glycyrrhiza uralensis Fisch. and provide theoretical basis for explaining pharmacodynamic principle of liquorice and discussing quality control of liquorice planting. [Method]The 6 elements Cu,Zn,Ca,Fe,Mg and Mn in roots of G.uralensis were extracted based on traditional decoction method and were separated into water-soluble state and suspension state by micro porous filtering film. The elements in water-soluble state were detected by flame atomic adsorption spectrophotometry (FAAS). [Result]The results showed that extractive rates of the elements were in the range of 1.71%-60.06%,and immerse-residue ratio in 0.018 3-1.682 0; the results also indicated that the immerse-residue ratio of Zn was biggest (1.68),Zn played an important medical role and might be considered as the best characteristic element in G.uralensis; the recoveries of the elements were ranged from 95.72% to 103.15% and relative standard deviations (RSD) were less than 2.38%. [Conclusion]Because of its high accuracy,FAAS method is feasible for analyzing primary speciation of microelements in G.uralensis.
基金
Supported by National Natural Science Foundation of China(30600806)
Science and Technology Project of Higher Education of Ningxia Hui Autonomous Region (NJ0626)~~
关键词
乌拉尔甘草
微量元素
火焰原子吸收光谱法
初级形态分析
Glycyrrhiza uralensis Fisch.
Microelement
Flame atomic adsorption sepctrophotometry (FAAS)
Primary speciation analysis