扁桃甙水解和极谱行为的研究

Studies on Hydrolysis and Polarographic Behavior of Amygdalin

通过测定产物间接测定扁桃甙,如用酶修饰电极测定产物氢氰酸和葡萄糖,其检测限均在10^(-5) mol/L。本文首次采用卷积伏安法研究了扁桃甙的水解以及产物苯甲醛的极谱行为,提高了扁桃甙的检测限。 1 仪器与试剂 XJP-821型新极谱仪(长春应用化学研究所、江苏电分析仪器厂),三电极体系。

Amygdalin is a non-active material. The polarographic study is based on the β-glucosidase-catalyzed hydrolysis of amygdalin to benzaldehyde. In a basic solution of injectio natrii chloridi the hy-drolysate of amygdalin exhibited a well defined reduction peak in a 2. 5th differential polarogram, the peak potential was - 1. 340V (Vs. Ag/AgCl). The current was proportional to amygdalin concentration in the range of 5. 9× 10-7 to 1. 4× 10-4mol/L. With increasing pH in the basic solution, peak potential negatively shifted. Scanning rate, surfactant temperature and cyclic polarogram show that the reduction wave is a irreversible process controlled by difusion. Specific activity of β-glucosidase was first determined by polarography. The effect of various enzyme concentration on peak current was observed.